Figure 3

Gene expression profiling after EphA3 modulation in LK63 and Reh cells. (a) Analysis of the deregulated genes in LK63 cells after EphA3 knockdown or in comparison with the EphA3-negative Reh cells, revealed a possibility that EphA3 affects CD79A/B and FYN expression to mediate the changes of expression of the genes in the network. (b) Flow cytometry of LK63/A3KD cells using IIIA4 and increased expression of CD79A/B demonstrated by increased IgM expression. (c) Western blots for FYN phosphorylation (Y530). (d) Western blot for total-FYN protein in LK63 and LK63/A3KD before or after EphA3 activation by ephrinA5-FC. The bands intensity was normalized to the Actin-control and the fold change compared with LK63 without EphA3 activation was calculated and shown under each lane.