Figure 2

Mesenchymal cells in LR-MDS display a distinct molecular signature characterized by cellular stress and inflammation. (a) Principal component analysis (PCA) on the transcriptomes of normal and LR-MDS mesenchymal cells. Patient numbers in a refer to LR-MDS patient IDs (Supplementary Table S1). (b) Example of GSEA plot revealing inflammatory response in the mesenchymal cells from LR-MDS. (c) Representative GSEA plot demonstrating deregulation of the gene set associated with cellular stress in response to UV in LR-MDS mesenchymal cells. Gene set size, NES and FDR values of each gene set is listed. (d) Number of CFU-F colonies formed by normal (n=3) or LR-MDS (n=3) CD271⁺ mesenchymal cells. (e) Representative images of cell clusters and colonies formed by mesenchymal cells from healthy control (left panel) and LR-MDS patients (right panel). (f) Comparison of significantly differentially expressed genes in FACS-purified CD271⁺ versus culture-expanded mesenchymal cells in LR-MDS. The total number of differentially regulated transcripts in each data set is indicated and the overlapping differentially regulated genes in the two data sets are listed. (g) Biologic processes significantly enriched (FDR<0.25) in FACS-purified CD271⁺ LR-MDS mesenchymal cells in comparison with expanded stromal cells defined by GO term analysis.**P<0.01. CFU-F, colony-forming unit - fibroblast; FDR, false discovery rate; NES, normalized enrichment score; UV, Ultraviolet.