Figure 4 | Leukemia

Figure 4

From: Heterodimerization of AML1/ETO with CBFβ is required for leukemogenesis but not for myeloproliferation

Figure 4

Colony forming potential of transduced progenitor populations. See overview in Supplementary Figure S4a. (a) AE9a displays higher replating efficiency compared to GFP control and CBFβ-binding deficient mutant AE9aNT. Colony-forming potential was measured by serial replating of sorted BM cells expressing IRES-GFP (control), AE9a-IRES-GFP (AE9a) and AE9aNT-IRES-GFP (AE9aNT) on semi-solid methylcellulose medium in seven days intervals over 5 weeks. Mean values and standard deviation (error bars) based on at least three independent experiments are shown. (nsNot significant, ***P<0.001). (b) Images of representative colonies of AE9a (upper panel) and AE9aNT (lower panel). (c) Analysis of cells in the colonies of AE9a (upper panel) and AE9aNT (lower panel) via Giemsa staining. While AE9a colonies consist of cells with immature morphology, AE9aNT colonies contain a higher number of mononuclear cells with a massive cytoplasmatic incorporation of methylcellulose (bars, 100 μm). Protein expression of AE9a and AE9aNT in cells isolated from methylcellulose was verified by western blot shown in Supplementary Figure S4b.

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