Figure 2
From: SETD2 and histone H3 lysine 36 methylation deficiency in advanced systemic mastocytosis
SETD2 protein and H3K36Me3 deficiency in SM. (a) representative western blot results for SETD2 protein and H3K36Me3 levels in SM patients as compared to a pool of healthy donors (HDs). One of three independent experiments is shown. (b and c) Box and whiskers plots of SETD2 and H3K36Me3 level estimates obtained by densitometric analysis of western blots. Median, interquartile range, minimum, maximum and outliers are indicated. SETD2 and H3K36Me3 signal intensities in single blots obtained from three individual experiments were normalized to those of beta-actin and H3 histone, respectively, and averaged. Normalized SETD2 and H3K36Me3 levels calculated in SM patients were then expressed in comparison to normalized SETD2 and H3K36Me3 levels detected in a pool of HDs, conventionally set to 1. The asterisks indicate that MCL and ASM had significantly lower levels of SETD2 protein (P<0.001 and P=0.002, respectively) and H3K36Me3 (P<0.001 and P=0.004, respectively) as compared to ISM.
