Figure 2 | Leukemia

Figure 2

From: Differential effects on gene transcription and hematopoietic differentiation correlate with GATA2 mutant disease phenotypes

Figure 2

Mutations in ZF2 domain perturb GATA2 activity and function. (a) Location of missense mutations in GATA2 ZF2 domain investigated in this study and their associated phenotypes. (b) GATA2 mutants differ in their DNA-binding affinity. Expression of full-length mGATA2 proteins (nuclear extracts (NE); 10 μg each) detected by western blotting with an anti-GATA2 antibody. (c) GATA2 NEs (10 μg) were bound to hGM-CSF and hTCRD enhancer oligonucleotide probes and western blotting electromobility shift assays were performed using anti-GATA2 antibody. Oligonucleotide probes with mutated GATA-binding site (Mut) for hGM-CSF or hTCRD enhancer were used as negative controls. ND, not determined. (d) DNA-binding affinity of hGATA2 ZF2 domain quantified by isothermal titration calorimetry.

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