Figure 3

GATA2 mutants display variable loss of transactivation capacity on known GATA2-responsive elements. (a) HEK293 cells were co-transfected with GATA2-responsive CD34 enhancer-LUC reporter and GATA2 (WT or mutants) expression constructs or EV. (b) Cos-7 cells were co-transfected with LYL1 promoter-LUC reporter and GATA2 expression constructs. (c) GATA2 expression constructs were co-transfected with CSF1R promoter-LUC into Cos-7 cells (white columns). Inset, a magnified view of the first nine conditions. The synergistic effect of GATA2 with PU.1 on the CSF1R promoter was investigated by co-transfecting GATA2 expression constructs with PU.1 (grey columns). Results are fold change compared with EV (±s.e.m., four independent biological replicates). Differences between WT (hatch) and mutants were calculated. ns: not significant, *P<0.05 and **P<0.01.