Figure 4

GATA2 mutants alter interaction with PU.1. (a) PU.1 was co-immunoprecipitated (co-IP) with GATA2 using anti-FLAG antibody (forward). (b) GATA2 was co-IP with PU.1 using anti-PU.1 antibody (reverse). Western blot analyses were performed with anti-GATA2 or anti-PU.1 antibody. gT354M and gC373R (*) show increased binding activity to PU.1.