Figure 1

RA-induced differentiation sensitizes NB4 cells to ER stress. (a) NB4 cells treated with 10 nM RA and 50 ng/ml Tm, alone or in combination as indicated, were analyzed for propidium iodide (PI) uptake as an indication of cell death (n=3±s.e.m.; Student’s T-test ***P-value<0.005; full analysis of variance (ANOVA) reported in Supplementary Table 1). (b) Morphological analysis of NB4 treated for 72 h. (c) The same cells were stained with anti-calreticulin (CARL) and anti-GRP78 (BiP) antibodies, and analyzed by confocal microscopy.