Figure 4

Attenuation of global translation protects differentiating NB4 cells from ER stress-induced death. (a) Propidium iodide (PI) uptake analysis of NB4 cells treated with RA and/or Tm, in the absence (nil) or presence of the PERK inhibitor GSK (300 nM), or of the GADD34 inhibitor Guanabenz (17 μM) (n=4±s.e.m.; Student’s T-test *P-value<0.05, **P-value<0.02; analysis of variance (ANOVA) presented in Supplementary Table 1). (b) Western blot analysis to assess the ratio between cleaved and uncleaved caspase-3. (c) Quantitative reverse transcriptase-PCR (qRT-PCR) for UPR target genes from samples treated as in (a) (n=3±s.e.m. at 24 h; n=2±s.e.m. at 72 h; ANOVA analysis in Supplementary Table 1).