Figure 1

FLT3 mutants and their subcellular localization with and without TKI treatment. (a) Schematic illustration of the FLT3 protein (NP_004110.2) and mutant constructs with indication of amino acid substitutions and insertion sequences (modified from Arreba-Tutusaus et al.39). (b) Immunofluorescence staining of FLT3-WT, FLT3 mutants or empty vector with or without AC220 treatment in transiently transfected U2OS cells. WGA (wheat germ agglutinin). Scale bar: 25 μm.