Figure 2
From: Activation of RHOA–VAV1 signaling in angioimmunoblastic T-cell lymphoma
Binding of SLP 76 and phosphorylated PLCγ1 as well as VAV1 and phospho VAV1 to G17V RHOA in Jurkat cells. Jurkatmock, JurkatWTRHOA and JurkatG17V cells were stimulated for 5 min with or without anti-CD3 antibody, followed by anti-mouse IgG antibody. Protein was immunoprecipitated from lysates using anti-Flag antibody and then immunoblotted with antibodies to Flag (RHOA), VAV1, phospho VAV1 (Tyr174), PLCγ1, phospho PLCγ1, and SLP76. β-Actin served as loading control. Asterisk (*) indicates G17V RHOA mutant. p-PLCγ1, phospho PLCγ1; p-VAV1, phospho VAV1.
