Figure 6
From: Activation of RHOA–VAV1 signaling in angioimmunoblastic T-cell lymphoma
Effect of G17V RHOA on NFAT activity or IL-2 expression in Jurkat cells. (a, b) Jurkat cells were transiently transfected with a reporter containing an NFAT response element (NFAT-RE) together with WT or G17V RHOA mutant cDNAs in the presence of absence of Dynabeads Human T-activator CD3/CD28. (a) NFAT activity in indicated samples. (b) Effect of dasatinib on NFAT activity. The mean±s.d. from triplicate samples is shown. (c, d) Jurkatmock, JurkatWTRHOA and JurkatG17V cells were stimulated in the presence or absence of Dynabeads Human T-activator CD3/CD28. (c) IL-2 gene expression based on real-time PCR. (d) Effect of dasatinib treatment on IL-2 expression in JurkatG17V cells. Cells were harvested at 3 h. The mean±s.d. from triplicate samples is shown.
