Table 1 Multiple myeloma NGF-MRD: 8- and 10-color antibody panels evaluated from the first (Version 1) to the final version (Version 5)

From: Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma

8-color panel version

Tube

PacB

PacO

FITC

PE

PerCPCy5.5

PECy7

APC

APCH7

1

1

CD45

CD138

CD38

CD56

CD27

CD19

CD117

CD81

 

2

CD45

CD138

CD38

   

CyIgκ

CyIgλ

   

HV500C

      

2

1

CD45

CD138

CD38

CD56

CD27

CD19

CD117

CD81

 

2

CD45

CD138

CD38

CD56

CD229

CD19

CyIgκ

CyIgλ

         

APC C750

3

1

CD45

CD138

CD38

CD56

CD27

CD19

CD117

CD81

 

2

CD45

CD138

CD38

CD56

CD229

CD19

CyIgκ

CyIgλ

  

HV450

       

4

1

CD138

CD27

CD38

CD56

CD45

CD19

CD117

CD81

 

2

CD138

CD27

CD38

CD56

CD45

CD19

CyIgκ

CyIgλ

  

BV421

BV510

      

5

1

CD138

CD27

CD38 (ME)

CD56

CD45

CD19

CD117

CD81

 

2

CD138

CD27

CD38 (ME)

CD56

CD45

CD19

CyIgκ

CyIgλ

10-color panel version

BV421

BV510

BV605

FITC

PE

PerCPCy5.5

PECy7

APC

APCAF700/AF700

APC C750

1

CD138

CD27

CyIgλ

CD38

CD56

CD45

CD19

CD117

CyIgκ

CD81

2

CD138

CD27

CD117

CD38

CD56

CD45

CD19

CyIgκ

 

CyIgλ

3

CD138

CD27

CD45

CD38 (ME)

CD56

CyIgλ

CD19

CD117

CD81

CyIgκ

  1. Abbreviations: APC, allophycocyanin; A700, alexa fluor 700; BV421, brilliant violet 421; BV510, brilliant violet 510; BV605, brilliant violet 605; Cy, cytoplasmic; C750, C750 dye; Cy5.5, cyanin5.5; Cy7, cyanin7; FITC, fluorescein isothiocyanate; HV450, Horizon V450; HV500C, Horizon V500C; H7, Hillite 7; Ig, immunoglobulin; MRD, minimal residual disease; ME, multi-epitope; PacB, pacific blue; PacO, pacific orange; PE, phycoerythrin; PerCP, peridinin–chlorophyll–protein. Changes from previous versions in markers or fluorochromes are highlighted in italics.
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