Figure 3
CLL architecture and temporal acquisition of driver alterations. (a) Graphical representation of the mutational and CNA status of the 406 untreated CLL cases studied. Cases are sorted based of their clonality as shown by the outer and innermost layers. The outer bar plot represents the number of genes mutated with clonal and subclonal, only clonal, subclonal-high and subclonal-low mutations for each case. The following inner layer represents the total number of driver alterations per case and the IGHV mutations. In the innermost layers, the basic genetic alterations and the total number of driver CNA are shown. (b) Representation of CLL driver alterations according to their classification as early, late or intermediate events. Temporal relationships between specific pairs of alterations are represented by arrows. (c) Evolutionary patterns observed in the longitudinal analysis regarding the driver CNA and gene mutations. Evol, evolution; mut, mutations (top). Mutations acquired during the course of the disease before or after treatment (bottom). The P-value of the Wilcox test applied to compare the number of mutations acquired in genes predicted as late events vs intermediate is shown.
