Figure 2

(a) Workflow, the results of the principal component analysis (PCA) performed on gene expression data obtained from DCs (from three different donors) transfected with NC or miR-29b and co-cultured with MM cells for 24 h, and the top 10 pathways perturbed by miR-29b enforced expression according to DAVID gene functional annotation tool. (b) (i) the magnitude of the modulation of genes belonging to the ‘Dendritic cell function’ canonical pathway affected by miR-29b enforced expression in DCs; (ii) the cellular functions most significantly affected by miR-29b overexpression (purple squares surround cell movement and chemotaxis pathways whose genes are mostly downregulated (blue shift) by miR-29b enforced expression); and (iii) ‘inflammatory/immunologic’ mediators network obtained by merging genes included in those functions and enriched by regulator genes, found to be modulated by miR-29b, with their respective fold changes. All these analyses have been performed through ingenuity pathways analysis software (IPA). (c) Anti-inflammatory switch of DCs according to the significant inflammatory/immune genes differentially expressed after miR-29b transfection as compared with inflammatory DCs from GSE40484 data set.