Figure 4
(a) Evaluation of the modulation of IL23 in DCs after miR-29b transfection and 48 h co-culture with MM cells, by qRT–PCR, cytokine production and secretion in the supernatant, and intracellular production through confocal microscopy. *P<0.05. (b) Overview of the in vivo synth-SCID-hu MM model and immunohistochemistry for the detection of DC-like cells (arrows) stained with anti human IL-23. (c) In the top part of the picture: workflow and qRT–PCR of RORC and IL17A (the major markers of Th17 polarization) performed on RNA extracted from autologous lymphocytes (naïve Th) after 72 h co-culture with 29b-DCs or NC-DCs previously co-cultured for 48 h with MM cells. *P<0.05. In the bottom part of the picture: representative dot-plots of Th17 (CD4+/CD161+) modulation after miR-29b enforced expression in DCs in the presence or absence of either apoptotic or necrotic U266 MM cell lines. The histograms represent the average of three independent experiments. *P<0.05.
