Figure 5
Intracellular localization of BCLAF1 determines its differential function. (a) IF analysis of BCLAF1 expression and localization in Usc and UmiR-194-5p cells upon 5 μm MS275 or SAHA treatment at 6 and 24 h. Black bar scale: 10 μm. (b) BCLAF1 and miR-194-5p expression by RNA-seq reads in human hematopoietic progenitors. (c) Expression levels of miR-194-5p and BCLAF1 analysed by real-time PCR during ex vivo expansion of lineage-depleted mouse bone morrow cells at T0, T1, T2 and T3 (0, 1, 2 and 4 days from bone marrow isolation); IF analysis of BCLAF1 localization during lineage differentiation of normal bone marrow mouse progenitors at T1, T2, T3 (1, 2, 4 days from bone marrow isolation) and again at T2 upon 24-h 5 μm MS275 or SAHA treatment. *P ⩽0.05.
