Figure 2
From: Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level
A schematic of the experimental procedures: rat neonatal cardiomyocytes are seeded into microwells microfabricated on PDMS-based biochips, which are placed inside 35-mm cell culture dishes with one chip per dish. The Rectangular (200 µm long and 25 µm wide) and circular (80 µm in diameter) microwells are designed for constructing aligned and random coculture models, respectively. A single rat mesenchymal stem cell is then deposited into each microwell at the space surrounded by the neonatal cardiomyocytes. Finally, immunostaining, single-cell PCR and patch clamp assays are performed on the biochips. MSC, mesenchymal stem cell; PCR, polymerase chain reaction; PDMS, polydimethylsiloxane.
