Figure 4
From: Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level
Confocal images of the immunostained cells in the microwells. (a) A portion of a biochip developed as an aligned coculture model; (b) an rMSC aligned with its surrounding cardiomyocytes inside a rectangular microwell; (c) a randomly spread rMSC inside a circular microwell; (d) an in vivo-like Cx 43 distribution between the rMSC and the surrounding cardiomyocytes; (e) an irregularly distributed Cx 43 expression; (f) cell fusion revealed by hybrid DiI-labeling, featuring the double nuclei and sarcomeric structures; (g) the corresponding phase-contrast image of the fused cell. The red circles represent the rMSC cell boundary traced from the corresponding phase images simultaneously obtained from the same confocal microscope. The white arrows point to Cx 43. rMSC, rat bone marrow mesenchymal stem cell.
