Figure 9

Images of microtubules in cells. (a) A conventional indirect immunofluorescence image. (b) The 3D STORM image of the same area with the depth information color-coded. (c) Confocal image. (d) The BaLM image of the same area. On comparison, it is apparent that stochastic readout microscopy can significantly enhance the resolution in both lateral and axial directions. Figure reprinted with permission: a and b, Ref. 96, ©2008 AAAS. BaLM, bleaching/blinking-assisted localization microscopy; 3D, three-dimensional; STORM, stochastic optical reconstruction microscopy.