Figure 3
From: Adaptive optical microscopy: the ongoing quest for a perfect image

Demonstrations of adaptive aberration correction in multiphoton microscopes before correction and after correction. (a) Two-photon fluorescence images of GFP-labelled Drosophila fruit fly brain, lateral section. (b) Third harmonic generation microscope image of a 5.5-day-old mouse embryo, axial section. (c) Projection of 3D rendering of two-photon fluorescence image stack of a mouse embryo labelled with GFP and DAPI. AC, after correction; BC, before correction; 3D, three-dimensional; DAPI, 4′,6-diamidino-2-phenylindole; GFP, Green fluorescent protein.