Figure 2 | Light: Science & Applications

Figure 2

From: Optical delivery of multiple opsin-encoding genes leads to targeted expression and white-light activation

Figure 2

Quantification results for the Lipofectamine-based transfection of different opsins. (a–d) ChR2-, (e–h) ReaChR-, and (i–l) White-opsin-transfected HEK293 cells. (a, e, i) Bright-field and (b, f, j) fluorescence images of HEK293 cells transfected with ChR2-YFP, ReaChR, and White-opsin, respectively, imaged using a 20× objective. Scale bar: 20 µm. (c, g, k) Representative intensity profiles of the opsin-reporter protein fluorescence along the lines drawn across the cells. (d, h, l) Quantitative comparison of expression in membrane and intracellular components in ChR2-, ReaChR-, and White-opsin-transfected cells. N = 6/opsin. Average ± S.D. No significant difference is observed between the membrane and the intracellular components. Flour., fluorescence.

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