Figure 3
Targeted laser-assisted transfection of single opsins. (a) Representative fluorescence image of targeted ChR2-YFP-optoporated HEK293 cells, (b) overlaid on the corresponding bright-field image showing highly packed cells in a monolayer, imaged using a 20× objective. (c) Representative fluorescence image of targeted HEK293 cells optoporated with ReaChR, (d) overlaid on the corresponding bright-field image showing cells in a monolayer, imaged using a 100× objective. Scale bar: 15 µm. (e, f) Representative fluorescence intensity profiles of the reporter proteins along the lines drawn across the cells (white lines in a, c). (g, h) Quantitative comparison of expression in the membrane and the intracellular components in cells optoporated with ChR2 and ReaChR. N = 5/opsin. Average ± S.D. *p < 0.01 difference between the membranes and intracellular components of opsin-expressing cells. Flour., fluorescence.
