Figure 4
Monitoring intracellular metabolic conversion. (a) Spontaneous Raman spectra of retinoic acid, all-trans retinol and triglyceride. (b) MCR output spectra of all-trans retinol treated cancer cells. The Raman peaks at 1580 and 1605 cm−1 indicated the presence of all-trans retinol and retinoic acid in cancer cells. The 1650 cm−1 peak resulted from acyl C=C bonds in lipid droplets. (c) The transmission image and MCR concentration map of all-trans retinol treated cancer cells. (d) The transmission image and MCR concentration map of the control group. The SRL spectroscopic images were averaged 10 times, which resulted in 320 µs pixel dwell time. Scale bar=10 µm. MCR, multivariate curve resolution; SRL, stimulated Raman loss.
