Figure 6
SRL spectral analysis of fast flowing objects. (a) A time-stack (∼300 ms) SRL image at 2914 cm−1 obtained by one-dimensional scan window of 50 µm. (b) Averaged time-lapsed spectra from the position of 32–37 µm in the time window of 30–66 ms. (c) Time-lapsed MCR concentration maps of PS and PMMA microspheres. (d) MCR output spectra and the corresponding Raman spectra. (e) A time-stack (∼300 ms) SRL image of flowing PC3 cells at 2874 cm−1. (f) Averaged time-lapsed spectra from the position of 25–30 µm in the time window of 27–63 ms. The pixel dwell time was 32 µs. MCR, multivariate curve resolution; PMMA, poly(methyl methacrylate); PS, polystyrene; SRL, stimulated Raman loss.
