Figure 4
From: Ultrafast laser-scanning time-stretch imaging at visible wavelengths
FACED-based time-stretch microscopy in an ultrafast microfluidic flow at 710 nm. (a, b) Images of Scenedesmus acutus in an ultrafast microfluidic flow captured by a a CMOS camera (15 000 f.p.s.) and b the FACED-based time-stretch microscope. The blur-free images reveal the subcellular structure, for example, vacuoles and long filaments (blue arrows), and the cell membrane of the non-pigmented Scenedesmus acutus, which is essentially detritus (yellow arrow). (c) Imagery of Scenedesmus acutus captured by the time-stretch microscope. (d, e) Time-stretch images of THP-1 and human red blood cells (RBCs) in an ultrafast microfluidic flow. Fine features, for example, blebs and the biconcave shapes of the RBCs, are visualized (blue arrows). (f, g) Imagery of THP-1 and RBCs captured by the time-stretch microscope. The imaging objectives used had numerical apertures of NA=0.25 for a and NA=0.66 for all time-stretch images. The scale bars represent 10 μm.
