Figure 3
R-TPM for DA. (a–d) Skeletonema marinoi and (e–h) Thalassiosira rotula. (a, e) Two mirror QPMs used to define the rotating angle retrieval rule. (b, f) Calculated tomograms, where the central slices are shown (the complete tomographic scan is provided in Supplementary Movies 5 and 6). Inset figures in the green boxes show the whole tomogram shapes, highlighting the volume occupied by diatoms given by the parameter V. (c, g) are obtained from (b, f), respectively, by applying a RI threshold to extract the 3D distribution of the chloroplasts and their volume (Vchl). (d, h) Fluorescence images used for comparison. Inset figures in the red boxes show the typical bright-field microscope images of the two diatoms.
