Figure 3

Resolution improvement of simulated data by deconvolution based on PEPSI’s PSF. (a) Degraded image of the object in Figure 1. The image is obtained by convolving the PSF (Figure 1c) with the object (Figure 1b) and adding Poisson noise (noise to signal ratio=0.014). Scale bar=32 μm. (b) Image reconstruction based on the speckles PSF estimation (Figure 1e) as initial input for iterative ML algorithm. (c) Averaged radial power spectrum of the reconstructed image (red) versus the degraded one (cyan), showing a resolution increase of the reconstructed image by having higher power at high resolvable frequencies.