Figure 9
Implementation of wavelength-scanning pixel super-resolution using a lens-based microscope. (a) Schematic of the optical setup. In this proof-of-concept experiment, a 10 × objective lens (NA=0.3) is used. The sample plane is scanned from 160 to 300 μm, defocused from the focal plane of the objective lens in 20-μm increments. The illumination wavelength is tuned between 500 and 521 nm with a step size of 3 nm. (b) Full FOV of a CMOS image sensor (pixel size: 3.75 μm) using the 10 × objective lens with a 0.38 × camera adaptor. (c) Lens-based image of a resolution test chart without wavelength-scanning pixel super-resolution. The half-pitch of the smallest resolved feature is 1.95 μm. (d) Lens-based image of the same resolution test chart with wavelength-scanning pixel super-resolution. The half-pitch of the smallest resolved feature is 0.78 μm.
