Figure 1
From: The contribution of PARs to inflammation and immunity to fungi
Fungi modulate par1 and par2 expression in vitro. (a) Purified Gr-1+ PMNs from wild-type mice were stimulated with Candida yeasts or Aspergillus conidia for 30 min before par1 and par2 gene expression was assessed by real-time PCR. The gapdh mRNA-normalized data were expressed as relative par mRNA.*P<0.05, stimulated vs. unstimulated (none) cells. (b) Surface staining by flow cytometric analysis of PMNs stimulated as in a. Black histograms refer to cells stained with control antibody. Numbers refer to the percentage of positive cells over total cells analyzed. (c) tlr2−/− and tlr4−/− PMNs were stimulated and assessed as in a. Bars are s.e. *P<0.05, stimulated vs. unstimulated cells. PMN, polymorphonuclear neutrophil.
