Figure 6
From: The contribution of PARs to inflammation and immunity to fungi
PAR2 attenuates inflammation and immunity to Aspergillus through TLR4. WT C57BL6 or tlr4−/− (a) mice (n=8, each group) were infected with A. fumigatus and treated with PAR2 agonists or antagonists (PAR2-AP and PAR2-ANT) as described in Methods. Periodic acid Schiff staining of sections from lungs (4 days after the infection) showing the presence of inflammatory cells (black arrows, inset). Bars indicate magnification. One representative of three experiments is shown. The production of oxidant (O2−) and MMP9 (b) was assessed in the lungs or stomach homogenates of infected mice (AU, arbitrary index of scanning densitometry). The bands show the active 92 kDa MMP9. Levels of O2− and MMP9 in uninfected controls were undetectable. *P<0.05, treated vs. untreated. MMP9, matrix metalloproteinase 9; WT, wild type.
