Figure 1
From: Identification of the predominant antigenic epitopes in intestinal flora in IBD
Step-by-step efficiency of cloning, small-scale protein expression and purification of 271 conserved proteins of E. coli. A total of 220 purified proteins with high and moderate yields were subjected to large-scale expression. Afterwards, 176 proteins could be purified with a minimum of 500 μg. Overall, 34 target proteins were subjected to Gateway recombination cloning to express fusion proteins with maltose binding protein, glutathione S-transferase, and N-utilizing substance A, respectively. Overall, 21 proteins fused to maltose binding protein were purified with a minimum of 500 μg. ECHH, E. coli-human-homologues; LUCA, Last Common Universal Ancestor
