Table 2 Apoptotic rate of epithelial HT-29/B6 cells in response to proinflammatory cytokines

From: Epithelial apoptosis is a prominent feature of the epithelial barrier disturbance in intestinal inflammation: effect of pro-inflammatory interleukin-13 on epithelial cell function

Cytokine

Concentration

Duration (h)

Apoptotic rate in %

Control

No addition

48

1.0±0.5 (histology)

IFNγ

100 U ml−1

48

1.1±0.5NS (histology)

IFNγ

1,000 U ml−1

48

1.2±0.4NS (histology)

IL-13

10 ng ml−1

48

5.6±0.9*** (histology)

Control

No addition

48

1.6±0.3 (FACS)

IL-13

10 ng ml−1

48

11.2±0.7*** (FACS)

IL-13+Z-VAD

10 ng ml−1+10 μm

48

1.1±0.2$ (FACS)

  1. FACS, fluorescence activated cell sorting; IFN, interferon; IL, interleukin; Z-VAD, N-benzyloxycarbonyl-Val-Ala-Asp.
  2. In HT-29/B6 cell monolayers, the influence of proinflammatory cytokines on apoptotic rate was determined.
  3. NS, not significantly different from control; ***P<0.001 vs. control; $P<00.1 vs. IL-13 addition without Z-VAD.
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