Table 1 Hemagglutination inhibition activity is induced in lung washings by lung immunization with reducing doses of influenza antigen and ISCOMATRIX adjuvant

From: Pulmonary delivery of ISCOMATRIX influenza vaccine induces both systemic and mucosal immunity with antigen dose sparing

  

HAI titer (geometric mean±s.d.)

  

Serum

Bronchoalveolar lavage

Route

Vaccine

Primary

Secondary

Tertiary

Primary

Secondary

Tertiary

S/C

15 μg flu

13±2

43±3

95±6

<10

<10

<10

Lung

15 μg flu+IMX

10±0

113±7

95±4

<10

17±4*

18±3*

Lung

5 μg flu+IMX

<10

78±4

174±3

<10

10±1

30±3*

Lung

1 μg flu+IMX

<10

23±2

147±3

<10

<10

<10

Lung

0.2 μg flu+IMX

<10

19±2

104±4

<10

<10

11±1

Lung

0.04 μg flu+IMX

<10

21±3

215±4

<10

<10

35±4*

Lung

0.008 μg flu+IMX

<10

11±2

23±3 #

<10

<10

12±2

  1. Sheep were immunized three times either subcutaneously (S/C) or via the lungs with influenza antigen (flu) with or without 100 μg ISCOMATRIX adjuvant (IMX). Sera and bronchoalveolar lavage collected after each immunization were analyzed for hemagglutination inhibition activity (HAI). Data, combined from two experiments, are expressed as the titer of sample that inhibited influenza agglutination of red blood cells. Groups contained n=8 sheep except the S/C group (n=12). Preimmunization samples were negative for HAI activity (not shown). For analysis of significance, log-transformed data were compared by ANOVA with Dunnett's post hoc analysis. #Significantly lower than subcutaneously immunized control (P<0.03). *Significantly higher than subcutaneously immunized control (P<0.002).
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