Table 3 Pulmonary immunization with ISCOMATRIX adjuvanted influenza vaccine induces antigen-specific memory proliferative responses in peripheral blood mononuclear cells

From: Pulmonary delivery of ISCOMATRIX influenza vaccine induces both systemic and mucosal immunity with antigen dose sparing

Pulmonary immunization

Median Stimulation Index (interquartile range) following restimulation with

Vaccine

Group size

5 μg influenza antigen

10 μg influenza antigen

0.04 μg influenza

n=8

1 (1–2)

1 (1–3)

0.04 μg influenza+100 μg IMX

n=8

6 (2–16)

10 (3–25)

  1. Sheep were immunized in their lungs with either 0.04 μg influenza antigen alone, or 0.04 μg influenza+100 μg ISCOMATRIX adjuvant. 1 week after the third immunization, peripheral blood was collected, and mononuclear cells cultured in 96-well plates with either media alone (control), or 5 or 10 μg/ml influenza antigen (restimulated). After 4 days, wells were pulsed with tritiated thymidine for 24 h. Radioactivity was measured and Stimulation Indices calculated by dividing the mean counts per minute (c.p.m.) for the restimulated group by the mean c.p.m. for the control group. A clear systemic antigen recall proliferative response was evident in sheep immunized via the pulmonary route with antigen plus adjuvant, but not with antigen alone.
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