Figure 5
Expression of activation/immune suppression markers in rectal biopsies. (a) Quantification of the expression of mRNAs for transforming growth factor (TGF)-β, (b) cytotoxic T lymphocyte antigen-4 (CTLA-4), (c) indoleamine 2,3-dioxygenase (IDO), and (d) FoxP3 in rectal mucosa from vaccinated (n=6) and mock-vaccinated (n=5) animals. Bars represent the average for each group±standard deviation. All values in figure (a)–(d) are normalized on the expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA. (e) Correlative analysis between logarithmic values for FoxP3 and CTLA4 mRNA in the rectal mucosa of vaccinated or (f) mock-vaccinated animals at weeks 12, 16, and 24 post-infection. (g) Kyn/Trp ratio measured by high-performance liquid chromatography (HPLC) in the plasma of vaccinated, and (h) mock-vaccinated animals at weeks 7, 12, and 16 and 24 post-infection. (i) Mean values of Kyn/Trp plasma ration in all animals overtime.
