Figure 4
P2 expression in outer membrane preparations of wild-type and P2-deficient nontypeable Haemophilus influenzae (NTHI). (a) Proteins were resolved on a 12% acrylamide gel and stained with Coomassie Blue. Molecular mass standards are noted in kDa on the left. Arrows indicate presence of the P2 protein in the wild-type strain; (1) wild-type and (2) P2-deficient mutant. (b) Immunoblot assay of wild-type and P2-deficient mutants. Whole-cell lysate was resolved on a 12% acrylamide gel and transferred to nitrocellulose. Blots were probed with antiserum to its homologous P2. Bound antibodies were detected with anti-mouse IgG-horseradish peroxidase (HRP). Molecular mass markers are noted in kDa on the left; (1) wild type and (2) P2-deficient mutant.
