Table 1 Levels of anti-rP2 serum IgG and saliva IgA from IN-immunized mice as determined by ELISA

From: Mucosal immunization of mice with recombinant OMP P2 induces antibodies that bind to surface epitopes of multiple strains of nontypeable Haemophilus influenzae

Immunization

Serum IgG (ng/ml) a

Saliva IgA (% IgA) b

rP213 (20 μg)

213.8±3.72

0.001±0.001

rP213 (50 μg)

869.1±168.5c

0.136±0.053c

Cholera toxin

269.1±34.22

0.001±0.001

Normal mouse serum

223.9±55.35

—d

rP223 (20 μg)

3855±1091c

0.137±0.021c

rP223 (50 μg)

5225±200.7c

0.096±0.023c

Cholera toxin

174.7±38.39

0.006±0.002

Normal mouse serum

133.2±30.45

0.012±0.006

rP254 (20 μg)

370.7±44.25

0.132±0.029c

rP254 (50 μg)

2076±255.6c

0.178±0.033c

Cholera toxin

261.0±50.26

0.006±0.001

Normal mouse serum

198.6±33.51

0.048±0.021

  1. ELISA, enzyme-linked immunosorbent assay; IgA, immunoglobulin A; IgG, immunoglobulin G; IN, intranasally; rP2, recombinant P2.
  2. aMean±s.e.m.
  3. bP2-specific IgA measured as a percent of total IgA (% total IgA±s.e.m.).
  4. cP-value <0.05 when compared with immunization with cholera toxin alone by Student's t-test.
  5. dValue below limit of detection.
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