Figure 3
From: MEP1A allele for meprin A metalloprotease is a susceptibility gene for inflammatory bowel disease
Strategy for Mep1a gene disruption on mouse chromosome 17. (a) Schematic diagram of a portion of the exon–intron structure of the WT and meprin αKO alleles. Exons (6–9) are represented as black boxes. The neomycin cassette derived from the targeting vector (Osdupdel: gift of O. Smithies) is depicted as a gray box in exon 7 of the KO allele. The 19-amino-acid consensus sequence for the catalytic center of astacin family metalloproteases is also shown. (b) Southern blot analysis of tail-derived genomic DNA. The probe used for Southern blotting detects two HindIII-generated fragments: 3.5 kb corresponding to the WT allele and 4.7 kb from the αKO allele. Lanes: −/−, meprin αKO DNA; +/+, WT DNA; +/−, meprin-α heterozygous DNA. WT, wild type.
