Figure 4
SIINFEKL-induced tolerance requires the presence of CD8+ T cells. CD8−/− mice were fed PBS, SIINFEKL, or wOVA followed by immunization and boost with wOVA. dLN cells were isolated and restimulated with 0, 10, or 100 μg ml−1 wOVA for 48 or 72 h. IFNγ, IL-2, and IL-17a protein levels in culture supernatants were determined by ELISA at the indicated time points; data are reported in pg ml−1±s.e. *P<0.05, **P<0.01, †P<0.001. The results are representative of five experiments (n=5 mice/group). dLN, draining lymph node; ELISA, enzyme-linked immunosorbent assay; PBS, phosphate-buffered saline; wOVA, whole OVA.
