Figure 5

Tolerance can be transferred to naive mice with CD8⁺ cells from SIINFEKL-fed mice, but not with CD8-depleted cells. CD8⁺ (5a) and CD8-depleted cells (5b) were isolated 3 h after the final feed from C57BL/6 mice fed PBS, SIINFEKL, or wOVA for 5 consecutive days. Naive mice received 2 × 10⁵ CD8⁺ cells (PBS n=8, SIIN n=10, and OVA n=10) or 1 × 10⁶ CD8-depleted cells (PBS n=7, SIIN n=5, and OVA n=10) IV by retro-orbital injection and were immunized 24 h later in the hindleg footpad with wOVA in CFA. dLN cells were isolated and restimulated with 0, 10, or 100 μg ml⁻¹ wOVA for 48 or 72 h. IFNγ and IL-17a protein levels in culture supernatants were determined by ELISA at the indicated time points; data are reported in pg ml⁻¹±s.e. **P<0.01, ^†P<0.001. The results are the combined data from two independent experiments. dLN, draining lymph node; ELISA, enzyme-linked immunosorbent assay; IV, intravenous; PBS, phosphate-buffered saline.

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