Figure 6
Fecal IgA is elevated in antigen-fed mice, whereas serum antibody levels remain unchanged. Blood and fecal pellets were collected from C57BL/6 mice fed PBS, SIINFEKL, OVA(323–339), or wOVA followed by immunization and boost with wOVA at the time of killing. (a) Serum total, IgG1 and IgG2a OVA-specific antibody titers were determined by ELISA. Data are reported as 50% reciprocal maximum binding titer. The results are representative of three experiments (n=5 mice/group). (b) Total IgA in fecal extracts was determined by ELISA. An IgA myeloma protein was incorporated on each plate as a standard; data are reported as ng of fecal IgA/mg of fecal pellet weight. *P<0.05. The results were compiled from five independent experiments (n=3–5 mice/group/experiment), each symbol represents a single mouse. ELISA, enzyme-linked immunosorbent assay; PBS, phosphate-buffered saline; wOVA, whole OVA.
