Figure 3
Breastfeeding-induced protection by ovalbumin (OVA)-exposed sensitized mothers does not require neither IgA nor transforming growth factor (TGF)-β in milk. Allergic airway disease in mice breastfed by sensitized mothers treated with anti-CCL28 mAb (a–c) or with anti-TGF-β mAb (d–f). Pups were breastfed by unexposed (gray bars) or OVA-exposed (black bars) sensitized mothers treated with isotypic control monoclonal antibody (mAb), or unexposed (dashed white bars) or OVA-exposed (dashed black bars) sensitized mothers treated with anti-CCL28 (a–c) or anti-TGF-β mAb (d–f). Data show cell number and eosinophils frequency in bronchoalveolar lavage (BAL) fluids (a and d), serum levels of OVA-specific IgE (b and e), and levels of IL-5 and IL-13 secreted by lung cells when incubated with OVA (c and f). Data are expressed as mean±s.e.m. of two experiments for anti-TGF-β experiment and one experiment for anti-CCL28 experiment with n=7 mice per group. **P<0.01; NS, P>0.05.
