Figure 6
In vitro induction of ovalbumin (OVA)-specific FoxP3+ Treg by milk-borne immune complexes. The milk from OVA-exposed or unexposed sensitized mice was harvested and either used immediately or fractionated on FPLC. Unfractionated milk (a and c) or the 500–2,000 kDa milk fraction (b and d) was incubated with bone marrow-derived dendritic cells and CFSE-labeled CD4+ T cells from DO11.10 T-cell receptor (TCR) transgenic RAG-2-deficient mice. Cells were harvested 5 days later and analyzed by FACS after staining with anti-CD3, anti-FoxP3, and KJ1.26 monoclonal antibodies (mAbs). Data show representative FACS profiles of one experiment out of four. The numbers indicate the frequency of cells that have progressed through the cell cycle (a and b) and the frequency of FoxP3+ cells among KJ1.26+ CD3+ cells (c and d).
