Figure 7
In vivo induction of ovalbumin (OVA)-specific FoxP3+ Treg by milk from OVA-exposed sensitized mothers. CD4+ cells from RAG-2-deficient DO11.10 (a–c) or Ly5.1+ OTII (d) TCR transgenic mice were injected into 1-day-old BALB/c (a–c) or wild-type or FcRn-deficient C57BL/6 pups (d). Mice were breastfed by unexposed or OVA-exposed sensitized mothers and mesenteric lymph nodes cells were harvested 10 days after weaning and analyzed by FACS. (a) Frequency of transferred OVA-specific T-cell receptor (TCR) transgenic T cells in BALB/c pups. Data show the frequency of KJ1.26+ cells among CD4+ T cells in individual mice (filled circles). The bars indicate the median values in one representative out of three experiments. (b and c) Cells from mice breastfed by unexposed or OVA-exposed sensitized mothers were analyzed by FACS. (b) CD25 expression. Data show the frequency of CD25+ cells among KJ1.26− and KJ1.26+ CD4+ cells in mice breastfed by unexposed (gray bars) and OVA-exposed (black bars) sensitized mothers (left panel) and representative FACS profiles (right panel). Data are expressed as mean±s.e.m. of one experiment out of three with n=6–8 mice per group in each experiment. (c) FoxP3 expression. Data show the frequency of FoxP3+ cells among KJ1.26− and KJ1.26+ CD4+ cells in mice breastfed by unexposed (gray bars) and OVA-exposed (black bars) sensitized mothers (left panel), and representative FACS profiles (right panel). Data are expressed as mean±s.e.m. of one experiment out of three with n=6–8 mice per group in each experiment. (d) FoxP3 expression in wild-type or FcRn-deficient C57BL/6 pups. Data show the frequency of FoxP3+ cells among Ly5.1− cells and donor Ly5.1+ cells in wild-type mice breastfed by unexposed (gray bars) or OVA-exposed (black bars) sensitized mothers and in FcRn-deficient mice breastfed by unexposed (dashed white bars) or OVA-exposed (dashed black bars) sensitized mothers (upper panel) and representative FACS profiles (lower panel). Data are expressed as mean±s.e.m. of one experiment out of two with n=6–8 mice per group in each experiment. ***P<0.001; **P<0.01.
