Figure 8
Breastfeeding-induced tolerance in anti-CD25 monoclonal antibody (mAb)-treated mice. BALB/c pups were breastfed by unexposed or ovalbumin (OVA)-exposed sensitized mothers. When 6-week-old, the offspring were treated with anti-CD25 or isotypic control mAb, sensitized with OVA 1 week later, and challenged with OVA aerosols. Data show the total number of cells and frequency of eosinophils in bronchoalveolar lavage (BAL) fluid (a), serum levels of OVA-specific IgE content (b), and levels of IL-5 and IL-13 secreted by lung cells when incubated with OVA (c) in mice breastfed by unexposed (gray bars) or OVA-exposed (black bars) sensitized mothers treated with an isotypic control mAb, and unexposed (dashed white bars) or OVA-exposed (dashed black bars) sensitized mothers treated with an anti-CD25 mAb. Data are expressed as mean±s.e.m. of two experiments with n=6–8 mice per group in each experiment ****P<0.0001; ***P<0.001; **P<0.01.
