Figure 2

CD11b⁺Gr1^intF4/80⁺ cells induced by lipopolysaccharide (LPS) administration are distinct from conventional lung dendritic cells (DCs). (a) CD11c⁺ cells were purified from the lung cells obtained from LPS-treated mice. Conventional DCs (cDCs) were identified as CD11c⁺ cells with a relatively low level of autofluorescence and side scatter and were used as APCs in experiments where needed. The purity of these cells was >95%. (b) 2 × 10⁵ cells (cDCs or CD11b⁺Gr1^intF4/80⁺ cells) were plated for 20 h and culture supernatants were analyzed for cytokine production. Values shown are mean±s.e.m., *P<0.01, **P<0.001. (c) Expression of co-stimulatory molecules detected by flow cytometry on the cDC and CD11b+Gr1^int F4/80+ populations. Dark lines represent expression of the indicated molecules, the light lines being background fluorescence determined by staining with appropriate isotype control antibodies. (d) Levels of nitric oxide (NO) in the supernatants of cell cultures. DC and Gr1^int/F4/80⁺ were isolated from the same LPS-treated mice as described before. Data shown are representative of two independent experiments and depict mean±s.d. *P<0.05.

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