Figure 5
Myeloid differentiation factor 88 (MyD88) dependence on the development of CD11b+Gr1int cells. (a) Bone marrow cells were cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and lipopolysaccharide (LPS) for 9 days and phenotypic analysis was performed using flow cytometry. The resulting cells from wild-type (WT) Balb/c mice were compared with those from MyD88-deficient (MyD88−/−) mice (left-hand panel), and those from WT C57BL/6 mice were compared with TRIF-deficient (TRIF−/−) mice (right-hand panel). (b) Estimation of fold induction of CD11b+Gr1int cells in response to LPS in the lungs of WT, TLR4−/−, and MyD88−/− mice. (c) MyD88−/− mice received HDM±LPS intratracheally (i.t.) as in Figure 4a. At 72 h after the final instillation, bronchoalveolar lavage (BAL) fluid and lung tissue samples were obtained. Total and differential counts of cells recovered in the BAL fluid (left-hand panel) and hematoxylin and eosin (H&E) staining of lung sections (right-hand panels) were performed. Values shown are mean±s.d., *P<0.05, **P<0.005. The concentration of interleukin-5 (IL-5) in lung homogenates was measured by multiplex assay and is presented as mean±s.e.m. ***P<0.001. The data shown are representative of two independent experiments.
