Figure 4
Increased spontaneous expression of interleukin (IL)-13 and IL-17 in CD11c-TL1A transgenic (tg) mice. (a) Relative mRNA expression of IL-13, IL-17, interferon-γ (IFN-γ), and IL-4 in mesenteric lymph node (mLN) cells of CD11c-TL1A tg and wild-type (wt) mice (7–24 weeks old) as determined by quantitative real-time PCR. (b) Relative mRNA expression of IL-13 and IL-17 in intestinal tissues of CD11c-TL1A tg and wt mice as determined by quantitative real-time PCR. Each data point represents the relative cytokine expression value of an individual mouse. Relative expression values were calculated from triplicate quantitative PCR samples. (c) Expression of IL-13, IL-17, and IFN-γ by re-stimulated CD4+ lamina propria lymphocytes (LPLs) isolated from the small intestine of CD11c-TL1A tg and wt mice. Each data point represents cytokine expression in cells isolated from an individual mouse.
