Figure 1
Airway hyperreactivity (AHR), airway inflammation, and antibody response in LACK (Leishmania analog of the receptors of activated C kinase)-sensitized and challenged mice. LACK-sensitized mice were challenged with (a, b) LACK or phosphate-buffered saline (PBS) or (c–f) LACK and analyzed (a) 5 days or (b–f) at the indicated days after challenge. (a) Airway resistance (R, left panel) and lung dynamic compliance (C, right panel) were measured in response to inhaled methacholine using a FlexiVent in anesthetized and tracheotomized mice. Data are shown for mice that have been challenged with LACK (filled squares) or PBS. (b) AHR was measured by whole-body plethysmography in response to inhaled methacholine (12 mg ml–1) at the indicated days following challenge with LACK (filled bars) or PBS (empty bars). (c–f) Bronchoalveolar lavage fluid (BALF) cells were counted and analyzed by fluorescence-activated cell sorting (FACS). (c) Total number of cells. (d) Number of eosinophils. (e) Number of lymphocytes. (f) Number of neutrophils. (g) Sera of LACK-sensitized and challenged mice were analyzed for LACK-specific IgE contents at the indicated days following LACK challenge. Data are expressed as mean±s.e.m.; n=10 mice per group. Two independent experiments were performed. *P<0.05; **P<0.01; NS, not significant.
